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Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification
Hung, Ivan1; Ge, Yuwei1,2; Liu, Xiaoli2; Liu, Mali2; Li, Conggang2; Gan, Zhehong1
2015-11-01
Source PublicationSOLID STATE NUCLEAR MAGNETIC RESONANCE
Volume72Pages:96-103
SubtypeArticle
AbstractExtended chemical shift anisotropy amplification (xCSA) is applied for measuring C-13/N-15 chemical shift anisotropy (CSA) of uniformly labeled proteins under magic-angle spinning (MAS). The amplification sequence consists of a sequence of R-pulses that repetitively interrupt MAS averaging of the CSA interaction. The timing of the pulses is designed to generate amplified spinning sideband manifolds which can be fitted to extract CSA parameters. The C-13/N-15 homonuclear dipolar interactions are not affected by the R-pulses due to the bilinear nature of the spin operators and are averaged by MAS in the xCSA experiment. These features make the constant evolution-time experiment suitable for measuring CSA of uniformly labeled samples. The incorporation of xCSA with multi-dimensional C-13/N-15 correlation is demonstrated with a GB1 protein sample as a model system for measuring C-13/N-15 CSA of all backbone (NH)-N-15, (13)CA and (CO)-C-13 sites. (C) 2015 Published by Elsevier Inc.
KeywordChemical Shift Anisotropy Csa Magic-angle Spinning Mas Spinning Sidebands Magic-angle Turning Mat Csa Amplification Extended Chemical Shift Modulation Protein Gb1
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1016/j.ssnmr.2015.09.002
WOS KeywordSOLID-STATE NMR ; NUCLEAR-MAGNETIC-RESONANCE ; ANGLE-SPINNING NMR ; MAGIC-ANGLE ; MICROCRYSTALLINE PROTEIN ; SPECTROSCOPY ; TENSORS ; SEQUENCES ; SPECTRA ; SAMPLES
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Physics ; Spectroscopy
WOS SubjectChemistry, Physical ; Physics, Atomic, Molecular & Chemical ; Physics, Condensed Matter ; Spectroscopy
WOS IDWOS:000365914000012
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/9075
Collection磁共振基础研究部
Affiliation1.Natl High Magnet Field Lab, Ctr Interdisciplinary Magnet Resonance, Tallahassee, FL 32310 USA
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Peoples R China
Recommended Citation
GB/T 7714
Hung, Ivan,Ge, Yuwei,Liu, Xiaoli,et al. Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification[J]. SOLID STATE NUCLEAR MAGNETIC RESONANCE,2015,72:96-103.
APA Hung, Ivan,Ge, Yuwei,Liu, Xiaoli,Liu, Mali,Li, Conggang,&Gan, Zhehong.(2015).Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification.SOLID STATE NUCLEAR MAGNETIC RESONANCE,72,96-103.
MLA Hung, Ivan,et al."Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification".SOLID STATE NUCLEAR MAGNETIC RESONANCE 72(2015):96-103.
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