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Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy
Li, Huihui1; An, Yanpeng1,2; Zhang, Lulu1; Lei, Hehua1; Zhang, Limin1; Wang, Yulan1,3; Tang, Huiru1,2
2013-12-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
Volume12Issue:12Pages:5520-5534
SubtypeArticle
AbstractInflammation is closely associated with pathogenesis of various metabolic disorders, cardiovascular diseases, and cancers. To understand the systems responses to localized inflammation, we analyzed the dynamic metabolic changes in rat plasma and urine associated with the carrageenan-induced self-limiting pleurisy using NMR spectroscopy in conjunction with multivariate data analysis. Fatty acids in plasma were also analyzed using GC-FID/MS with the data from clinical chemistry and histopathology as complementary information. We found that in the acute phase of inflammation rats with pleurisy had significantly lower levels in serum albumin, fatty acids, and lipoproteins but higher globulin level and larger quantity of pleural exudate than controls. The carrageenan-induced inflammation was accompanied by significant metabolic alterations involving TCA cycle, glycolysis, biosyntheses of acute phase proteins, and metabolisms of amino acids, fatty acids, ketone bodies, and choline in acute phase. The resolution process of pleurisy was heterogeneous, and two subgroups were observed for the inflammatory rats at day-6 post treatment with different metabolic features together with the quantity of pleural exudate and weights of thymus and spleen. The metabolic differences between these subgroups were reflected in the levels of albumin and acute-phase proteins, the degree of returning to normality for multiple metabolic pathways including glycolysis, TCA cycle, gut microbiota functions, and metabolisms of lipids, choline and vitamin B-3. These findings provided some essential details for the dynamic metabolic changes associated with the carrageenan-induced self-limiting inflammation and demonstrated the combined NMR and GC-FID/MS analysis as a powerful approach for understanding biochemical aspects of inflammation.
KeywordCarrageenan-injection Pleurisy Inflammation Metabonomics Fatty Acids
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1021/pr400440d
WOS KeywordACUTE-PHASE RESPONSE ; SPINNING H-1-NMR SPECTROSCOPY ; POLYUNSATURATED FATTY-ACIDS ; MULTIVARIATE DATA-ANALYSIS ; NITRIC-OXIDE SYNTHASE ; HUMAN-BLOOD-PLASMA ; ACUTE-INFLAMMATION ; GUT MICROBIOTA ; RESOLUTION ; CYCLOOXYGENASE
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000328231300015
Citation statistics
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/786
Collection磁共振应用研究部
Affiliation1.Univ Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn Biospect & Metabol, Shanghai 200433, Peoples R China
3.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310058, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Li, Huihui,An, Yanpeng,Zhang, Lulu,et al. Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(12):5520-5534.
APA Li, Huihui.,An, Yanpeng.,Zhang, Lulu.,Lei, Hehua.,Zhang, Limin.,...&Tang, Huiru.(2013).Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy.JOURNAL OF PROTEOME RESEARCH,12(12),5520-5534.
MLA Li, Huihui,et al."Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy".JOURNAL OF PROTEOME RESEARCH 12.12(2013):5520-5534.
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