APM OpenIR
Enhancement of hydrogen peroxide tolerance of lipase LipA from Bacillus subtilis using semi-rational design
Jiang, Hong1,2,4; Jia, Wenjing1,2,4; Duan, Mojie5; Lin, Hong1,2,4; Huang, Jianzhong1,2,4; Li, Xin1,2,4; Shu, Zhengyu1,2,3,4
2020-07-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume159Pages:9
AbstractIn many application fields, microbial lipases have to coexist with hydrogen peroxide in the same reaction system and it is necessary to construct hydrogen peroxide-tolerant lipase mutant variants. Mass changes between native amino acids and the oxidized amino acids by hydrogen peroxide were determined using mass spectrometry in early research. Based on the known 3D structure information and mass spectrometric analysis data, an extremely small library of Bacillus subtilis lipase LipA consisting of twenty-six mutant variants was constructed and screened. From the mutation library, total twenty-one lipase LipA mutant variants improved hydrogen peroxide tolerance. Compared with that of lipase LipA, the C-50 value and t(1/2) value of the best mutant variant, LipA-Trp(42)Ala/Met(134)Glu was increased by 9.1-fold and 9.5-fold, respectively.
KeywordBacillus subtilis lipase A Hydrogen peroxide tolerance Oxidation Stability
Funding OrganizationNational Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council
DOI10.1016/j.bej.2020.107590
WOS KeywordHORSERADISH-PEROXIDASE ; METHIONINE RESIDUES ; AMINO-ACIDS ; OLD ENZYMES ; OXIDATION ; PROTEIN ; STABILITY ; MASS ; THERMOSTABILITY ; IDENTIFICATION
Language英语
Funding ProjectNational Natural Science Funds of China[31870787] ; National Natural Science Funds of China[31370802] ; Natural Science Funds of Fujian Province, China[2017J01441] ; China Scholarship Council
Funding OrganizationNational Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Funds of China ; National Natural Science Funds of China ; Natural Science Funds of Fujian Province, China ; Natural Science Funds of Fujian Province, China ; China Scholarship Council ; China Scholarship Council
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000537604100014
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/22811
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorDuan, Mojie; Huang, Jianzhong; Shu, Zhengyu
Affiliation1.Fujian Normal Univ, Natl & Local United Engn Res Ctr Ind Microbiol &, Minist Educ, Fuzhou 350117, Peoples R China
2.Fujian Normal Univ, Engn Res Ctr Ind Microbiol, Minist Educ, Fuzhou 350117, Peoples R China
3.Fujian Normal Univ, Prov Univ Key Lab Cellular Stress Response & Meta, Fuzhou 350117, Peoples R China
4.Fujian Normal Univ, Coll Life Sci, Qishan Campus, Fuzhou 350117, Peoples R China
5.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Hong,Jia, Wenjing,Duan, Mojie,et al. Enhancement of hydrogen peroxide tolerance of lipase LipA from Bacillus subtilis using semi-rational design[J]. BIOCHEMICAL ENGINEERING JOURNAL,2020,159:9.
APA Jiang, Hong.,Jia, Wenjing.,Duan, Mojie.,Lin, Hong.,Huang, Jianzhong.,...&Shu, Zhengyu.(2020).Enhancement of hydrogen peroxide tolerance of lipase LipA from Bacillus subtilis using semi-rational design.BIOCHEMICAL ENGINEERING JOURNAL,159,9.
MLA Jiang, Hong,et al."Enhancement of hydrogen peroxide tolerance of lipase LipA from Bacillus subtilis using semi-rational design".BIOCHEMICAL ENGINEERING JOURNAL 159(2020):9.
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