APM OpenIR
Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model
Luo, Dan1; Miao, Yuanjiu1; Ke, Xianliang1; Tan, Zhongyuan1; Hu, Chun2; Li, Penghui1; Wang, Ting1,3; Zhang, Yuan1; Sun, Jianhong1; Liu, Yan1; Wang, Hanzhong1; Zheng, Zhenhua1
2020-05-29
Source PublicationVIROLOGICA SINICA
ISSN1674-0769
Pages14
AbstractZika virus (ZIKV) is emerging as a significant pathogen worldwide and may cause severe neurological disorders such as fetal microcephaly and Guillain-Barre syndrome. No drug or listed vaccines are currently available for preventing ZIKV infection. As a major target of neutralizing, ZIKV envelop (E) protein usually used for vaccine development. Nevertheless, the immunogenicity of ZIKV envelop (E) protein expressed by baculovirus display system has never been assessed. In this study, we reported a new strategy for surface display of ZIKV E protein by a recombinant baculovirus vector derived from Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and assessed its immunogenicity in mice. We produced recombinant fusion ZIKV E protein linked with signal peptide (SP) and transmembrane domain (TM) of AcMNPV GP64. The results showed that the recombinant protein was easy to produce by baculovirus display system. BALB/c mice immunized with this recombinant E protein developed ZIKV specific serum antibodies. The anti-E protein sera from the mice were able to effectively neutralize ZIKV in vitro. More importantly, AG6 (IFN-alpha/beta and IFN-gamma receptor deficient) mice immunized with recombinant E protein were protected against lethal ZIKV challenge. Together, these findings demonstrated that the recombinant E protein displayed by baculovirus can be conveniently prepared and displayed good immunogenicity in immunized mice. It is a promising practical approach for prompting the development of vaccine and related immunology research.
KeywordZika virus (ZIKV) Envelope protein Baculovirus Immunogenicity Neutralizing antibody Viral challenge
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS
DOI10.1007/s12250-020-00238-x
WOS KeywordIMMUNITY ; INSECT ; TRANSMISSION ; ANTIBODIES ; VECTORS ; MICE
Language英语
Funding ProjectNational Key R&D Program of China[2016YFD0500406] ; National Natural Science Foundation of China (NSFC)[81672036] ; Youth Innovation Promotion Association of CAS[2016302]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000536322000001
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/22785
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorZheng, Zhenhua
Affiliation1.Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China
2.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Comp Ctr, Wuhan 430030, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
Recommended Citation
GB/T 7714
Luo, Dan,Miao, Yuanjiu,Ke, Xianliang,et al. Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model[J]. VIROLOGICA SINICA,2020:14.
APA Luo, Dan.,Miao, Yuanjiu.,Ke, Xianliang.,Tan, Zhongyuan.,Hu, Chun.,...&Zheng, Zhenhua.(2020).Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model.VIROLOGICA SINICA,14.
MLA Luo, Dan,et al."Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model".VIROLOGICA SINICA (2020):14.
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