APM OpenIR
Functions of lactate in the brain of rat with intracerebral hemorrhage evaluated with MRI/MRS and in vitro approaches
Liu, Yue1; Yang, Shusheng2,3; Cai, Erli2; Lin, Li2,3; Zeng, Peng2; Nie, Binbin4; Xu, Fuqiang1,5,6,7; Tian, Qing2; Wang, Jie1,7,8
2020-06-02
Source PublicationCNS NEUROSCIENCE & THERAPEUTICS
ISSN1755-5930
Pages14
AbstractIntroduction Lactate accumulation in the brain is caused by the anaerobic metabolism induced by ischemic damages, which always accompanies intracerebral hemorrhages (ICH). Our former findings showed that microglia's movement was always directly toward hemorrhagic center with the highest lactate concentration, and penumbra area has the largest density of compactly arrayed microglia. However, the relationship between microglia and lactate concentration has not been well documented. Methods Cerebral hemorrhage model was successfully achieved by injecting collagenase VII (causing stabile localized bleeding) in CPu (striatum) of SD rats. Emodin was used as a potential therapeutic for ICH. The function of the lactate was examined with in vitro culture studies. Then, the effect of lactate on the proliferation, cell survival, migration, and phagocytosis property of microglia was investigated by in vitro culture studies. Results Lactate accumulation was observed with in vivo MRS method, and its concentration was monitored during the recovery of ICH and treatment of emodin. Lactate concentration significantly increased in the core and penumbra regions of hemorrhagic foci, and it decreased after the treatment of emodin. The in vitro culture study was verified that lactate was beneficial for the proliferation, cell survival, migration, and phagocytosis property of the microglia. Conclusion Results from in vitro verification study, investigations from the recovery of ICH, and treatment of emodin verify that lactate plays an important role during the recovery of ICH. This could provide a novel therapeutic approach for ICH.
Keywordemodin hemorrhages in vivo MRS lactate microglia
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences
DOI10.1111/cns.13399
WOS KeywordMETABOLIC ALTERATIONS ; ENERGY-METABOLISM ; MICRODIALYSIS ; ASPARTATE ; GLUTAMATE ; CORTEX ; OXYGEN
Language英语
Funding ProjectNatural Science Foundation of China[91539112] ; Natural Science Foundation of China[31800876] ; Natural Science Foundation of China[31771193] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[Y6Y0021004]
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Natural Science Foundation of China ; Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences
WOS Research AreaNeurosciences & Neurology ; Pharmacology & Pharmacy
WOS SubjectNeurosciences ; Pharmacology & Pharmacy
WOS IDWOS:000537090800001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/22712
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorTian, Qing; Wang, Jie
Affiliation1.Chinese Acad Sci, Innovat Acad Precis Measurement Sci & Technol, Wuhan Inst Phys & Math,State Key Lab Magnet Reson, Wuhan Ctr Magnet Resonance,Key Lab Magnet Resonan, Wuhan 430071, Peoples R China
2.Huazhong Univ Sci & Technol, Inst Brain Res, Sch Basic Med, Dept Pathol & Pathophysiol, Hangkong Rd 13, Wuhan 430030, Peoples R China
3.Hubei Univ Chinese Med, Cell Mol Biol Lab Basic Med Coll, Wuhan, Peoples R China
4.Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Radiat & Nucl Energy Technol, Beijing, Peoples R China
5.Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Wuhan, Peoples R China
6.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligent Technol, Shanghai, Peoples R China
7.Univ Chinese Acad Sci, Beijing, Peoples R China
8.2nd Hosp Shijiazhuang, Hebei Prov Key Lab Basic Med Diabet, Shijiazhuang, Hebei, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yue,Yang, Shusheng,Cai, Erli,et al. Functions of lactate in the brain of rat with intracerebral hemorrhage evaluated with MRI/MRS and in vitro approaches[J]. CNS NEUROSCIENCE & THERAPEUTICS,2020:14.
APA Liu, Yue.,Yang, Shusheng.,Cai, Erli.,Lin, Li.,Zeng, Peng.,...&Wang, Jie.(2020).Functions of lactate in the brain of rat with intracerebral hemorrhage evaluated with MRI/MRS and in vitro approaches.CNS NEUROSCIENCE & THERAPEUTICS,14.
MLA Liu, Yue,et al."Functions of lactate in the brain of rat with intracerebral hemorrhage evaluated with MRI/MRS and in vitro approaches".CNS NEUROSCIENCE & THERAPEUTICS (2020):14.
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