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High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues
Beckonert, Olaf1; Coen, Muireann1; Keun, Hector C.1; Wang, Yulan2; Ebbels, Timothy M. D.1; Holmes, Elaine1; Lindon, John C.1; Nicholson, Jeremy K.1
2010
Source PublicationNATURE PROTOCOLS
Volume5Issue:6Pages:1019-1032
Contribution Rank第二
SubtypeArticle
AbstractMetabolic profiling, metabolomic and metabonomic studies require robust study protocols for any large-scale comparisons and evaluations. Detailed methods for solution-state NMR spectroscopy have been summarized in an earlier protocol. this protocol details the analysis of intact tissue samples by means of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy and we provide a detailed description of sample collection, preparation and analysis. Described here are (1)H NMR spectroscopic techniques such as the standard one-dimensional, relaxation-edited, diffusion-edited and two-dimensional J-resolved pulse experiments, as well as one-dimensional (31)p NMR spectroscopy. these are used to monitor different groups of metabolites, e. g., sugars, amino acids and osmolytes as well as larger molecules such as lipids, non-invasively. Through the use of NMR-based diffusion coefficient and relaxation times measurements, information on molecular compartmentation and mobility can be gleaned. the NMR methods are often combined with statistical analysis for further metabonomics analysis and biomarker identification. the standard acquisition time per sample is 8-10 min for a simple one-dimensional (1)H NMR spectrum, giving access to metabolite information while retaining tissue integrity and hence allowing direct comparison with histopathology and MRI/MRS findings or the evaluation together with biofluid metabolic-profiling data.
Department生物波谱及代谢组学
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Subject Area波谱分析
DOI10.1038/nprot.2010.45
WOS KeywordMAGNETIC-RESONANCE-SPECTROSCOPY ; HR-MAS SPECTROSCOPY ; PULSED-FIELD GRADIENTS ; H-1-NMR SPECTROSCOPY ; PATTERN-RECOGNITION ; BIOPSY SAMPLES ; IN-VIVO ; ALPHA-NAPHTHYLISOTHIOCYANATE ; TEMPERATURE CALIBRATION ; INDUCED HEPATOTOXICITY
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000278354700005
Citation statistics
Cited Times:232[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/2058
Collection2011年以前论文发表(包括2011年)
Affiliation1.Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Surg & Canc, London, England
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Beckonert, Olaf,Coen, Muireann,Keun, Hector C.,et al. High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues[J]. NATURE PROTOCOLS,2010,5(6):1019-1032.
APA Beckonert, Olaf.,Coen, Muireann.,Keun, Hector C..,Wang, Yulan.,Ebbels, Timothy M. D..,...&Nicholson, Jeremy K..(2010).High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues.NATURE PROTOCOLS,5(6),1019-1032.
MLA Beckonert, Olaf,et al."High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues".NATURE PROTOCOLS 5.6(2010):1019-1032.
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