APM OpenIR
Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor
Guo Xingdong1; Yan Chengsong1; Li Hua1; Huang Wenmao2; Shi Xiaoshan1; Huang Min1; Wang Yingfang1; Pan Weiling1; Cai Mingjun3; Li Lunyi1; Wu Wei1; Bai Yibing1; Zhang Chi1; Liu Zhijun1; Wang Xinyan1; Zhang Xiaohui F4; Tang Chun5; Wang Hongda3; Liu Wanli6; Ouyang Bo1; Wong Catherine C1; Cao Yi2; Xu Chenqi1
2017-01-01
Source PublicationCell Research
ISSN1001-0602
Volume27Issue:4Pages:505
AbstractT-cell receptor-CD3 complex (TCR) is a versatile signaling machine that can initiate antigen-specific immune responses based on various biochemical changes of CD3 cytoplasmic domains, but the underlying structural basis remains elusive. Here we developed biophysical approaches to study the conformational dynamics of CD3 epsilon cytoplasmic domain (CD3 epsilon(CD)). At the single-molecule level, we found that CD3 epsilon(CD) could have multiple conformational states with different openness of three functional motifs, i.e., ITAM, BRS and PRS. These conformations were generated because different regions of CD3 epsilon(CD) had heterogeneous lipid-binding properties and therefore had heterogeneous dynamics. Live-cell imaging experiments demonstrated that different antigen stimulations could stabilize CD3 epsilon(CD) at different conformations. Lipid-dependent conformational dynamics thus provide structural basis for the versatile signaling property of TCR.
Language英语
Funding Project[Chinese Academy of Sciences (Strategic Priority Research Program)] ; [National Natural Science Foundation of China] ; [Ministry of Science and Technoloy of China] ; [China Postdoctoral Science Foundation]
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/16218
Collection中国科学院武汉物理与数学研究所
Affiliation1.中国科学院
2.南京大学
3.中国科学院长春应用化学研究所
4.理海大学
5.中国科学院武汉物理与数学研究所
6.清华大学
Recommended Citation
GB/T 7714
Guo Xingdong,Yan Chengsong,Li Hua,et al. Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor[J]. Cell Research,2017,27(4):505.
APA Guo Xingdong.,Yan Chengsong.,Li Hua.,Huang Wenmao.,Shi Xiaoshan.,...&Xu Chenqi.(2017).Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor.Cell Research,27(4),505.
MLA Guo Xingdong,et al."Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor".Cell Research 27.4(2017):505.
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