WIPM OpenIR
Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion
Liu, Caixiang1; Chen, Fangfang2,3; Zhang, Jingtao1; Liu, Laixing4; Lei, Hehua1; Li, Heping3; Wang, Yulan1,5; Liao, Yu-Cai3; Tang, Huiru1,6,7
2019-09-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume18Issue:9Pages:3317-3327
AbstractFusarium head blight (FHB) mainly resulting from Fusarium graminearum (Fg) Schwabe is a notorious wheat disease causing huge losses in wheat production globally. Fg also produces mycotoxins, which are harmful to human and domestic animals. In our previous study, we obtained two Fg mutants, TPS1(-) and TPS2(-), respectively, with a single deletion of trehalose 6-phosphate synthase (TPS1) and trehalose 6-phosphate phosphatase (TPS2) compared with the wild type (WT). Both mutants were unable to synthesize trehalose and produced fewer mycotoxins. To understand the other biochemical changes induced by TPS gene deletion in Fg, we comprehensively analyzed the metabolomic differences between TPS- mutants and the WT using NMR together with gas chromatography-flame ionization detection/mass spectrometry. The expression of some relevant genes was also quantified. The results showed that TPS1(-) and TPS2(-) mutants shared some common metabolic feature such as decreased levels for trehalose, Val, Thr, Lys, Asp, His, Trp, malonate, citrate, uridine, guanosine, inosine, AMP, C10:0, and C16:1 compared with the WT. Both mutants also shared some common expressional patterns for most of the relevant genes. This suggests that apart from the reduced trehalose biosynthesis, both TPS1 and TPS2 have roles in inhibiting glycolysis and the tricarboxylic acid cycle but promoting the phosphopentose pathway and nucleotide synthesis; the depletion of either TPS gene reduces the acetyl-CoA-mediated mycotoxin biosynthesis. TPS2(-) mutants produced more fatty acids than TPS1(-) mutants, suggesting different roles for TPS1 and TPS2, with TPS2(-) mutants having impaired trehalose biosynthesis and trehalose 6-phosphate accumulation. This may offer opportunities for developing new fungicides targeting trehalose biosynthesis in Fg for FHB control and mycotoxin reduction in the FHB-affected cereals.
KeywordFusarium graminearum TPS1(-) TPS2(-) metabonomics NMR GC-FID/MS
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China
DOI10.1021/acs.jproteome.9b00259
WOS KeywordTREHALOSE 6-PHOSPHATE PHOSPHATASE ; STRESS-RESPONSE ; TREHALOSE-6-PHOSPHATE PHOSPHATASE ; HEAD BLIGHT ; WHEAT ; SYSTEMS ; BIOSYNTHESIS ; RESISTANCE ; EXPRESSION ; CLONING
Language英语
Funding ProjectNational Natural Science Foundation of China[31600240] ; National Natural Science Foundation of China[31272004] ; National Natural Science Foundation of China[31201475] ; National Basic Research Program of China[2013CB127801] ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University)[KF201909] ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China[K201815]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Open Research Fund of State Key Laboratory of Hybrid Rice (Wuhan University) ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China ; Science Research Foundation of Wuhan Institute of Technology of Hubei Province of China
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000485089100009
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/14932
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorWang, Yulan; Liao, Yu-Cai; Tang, Huiru
Affiliation1.Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
2.Hubei Univ, Sch Life Sci, Hubei Collaborat Innovat Ctr Green Transformat Bi, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Hubei, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Hubei, Peoples R China
4.Wuhan Inst Technol, Sch Management, Wuhan 430205, Hubei, Peoples R China
5.Nanyang Technol Univ, Singapore Phenome Ctr, Lee Kong Chian Sch Med, Sch Biol Sci, Singapore, Singapore
6.Fudan Univ, Zhongshan Hosp, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
7.Fudan Univ, Sch Life Sci Metabol & Syst Biol, Lab Human Phenome Inst, Collaborat Innovat Ctr Genet & Dev, Shanghai 200433, Peoples R China
Recommended Citation
GB/T 7714
Liu, Caixiang,Chen, Fangfang,Zhang, Jingtao,et al. Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(9):3317-3327.
APA Liu, Caixiang.,Chen, Fangfang.,Zhang, Jingtao.,Liu, Laixing.,Lei, Hehua.,...&Tang, Huiru.(2019).Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion.JOURNAL OF PROTEOME RESEARCH,18(9),3317-3327.
MLA Liu, Caixiang,et al."Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion".JOURNAL OF PROTEOME RESEARCH 18.9(2019):3317-3327.
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