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Quantitative analysis of 10 classes of phospholipids by ultrahigh-performance liquid chromatography tandem triple-quadrupole mass spectrometry
Huang, Qingxia1,2; Lei, Hehua1; Dong, Manyuan1,2; Tang, Huiru3,4; Wang, Yulan5
2019-07-07
Source PublicationANALYST
ISSN0003-2654
Volume144Issue:13Pages:3980-3987
AbstractPhospholipids are the main constituents of biological membranes and their biological function has been increasingly recognized. Therefore, there is an unmet need to develop methods capable of quantifying a wide range of phospholipids with high sensitivities and high throughput. We employed an ultrahigh-performance liquid chromatography system coupled to a triple-quadrupole mass spectrometer (UHPLC-MS) and developed a method that can quantitatively analyze 10 major classes of phospholipids in biological samples in 11 min. These are phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidic acid, lysophosphatidylcholine and lysophosphatidylethanolamine. The limit of detection (LOD) and limit of quantitation (LOQ) are 0.04-33 pmol mL(-1) and 0.1-110 pmol mL(-1), respectively. The method takes three steps: first and second steps identified phospholipid structures in a mixture containing aliquots of all the samples using the combinations of multiple reaction monitoring (MRM), product ion scan and retention time in the positive and negative ion modes. These steps enabled the identification of phospholipids present in the samples and provided information on efficient sample analysis in the final step of sample quantitative analysis. We have developed fast and sensitive label-free quantitation with normalization of the acyl chain length to achieve more accurate quantification. The method developed was applied to analyze 6 different biological samples (plasma, cells and tissues) for applicability validation, where a total of 308 phospholipid species across 10 phospholipid classes were identified and 295 phospholipid species were quantified. The method is highly efficient, sensitive, and is universally applicable.
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China
DOI10.1039/c9an00676a
WOS KeywordIONIZATION ; LIPIDOMICS ; PHOSPHATIDYLCHOLINES ; IDENTIFICATION ; EXTRACTION ; SEPARATION
Language英语
Funding ProjectNational Key R&D Program of China[2017YFC0906800] ; National Science Foundation of China[21675169]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000474584300006
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/14622
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorWang, Yulan
Affiliation1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Fudan Univ, Zhongshan Hosp, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
4.Fudan Univ, Human Phenome Inst, Lab Metabon & Syst Biol, Sch Life Sci, Shanghai 200433, Peoples R China
5.Nanyang Technol Univ, Singapore Phenome Ctr, Lee Kong Chian Sch Med, Sch Biol Sci, Singapore, Singapore
Recommended Citation
GB/T 7714
Huang, Qingxia,Lei, Hehua,Dong, Manyuan,et al. Quantitative analysis of 10 classes of phospholipids by ultrahigh-performance liquid chromatography tandem triple-quadrupole mass spectrometry[J]. ANALYST,2019,144(13):3980-3987.
APA Huang, Qingxia,Lei, Hehua,Dong, Manyuan,Tang, Huiru,&Wang, Yulan.(2019).Quantitative analysis of 10 classes of phospholipids by ultrahigh-performance liquid chromatography tandem triple-quadrupole mass spectrometry.ANALYST,144(13),3980-3987.
MLA Huang, Qingxia,et al."Quantitative analysis of 10 classes of phospholipids by ultrahigh-performance liquid chromatography tandem triple-quadrupole mass spectrometry".ANALYST 144.13(2019):3980-3987.
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