APM OpenIR
Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage
Guan, Zeyuan1,2; Pei, Kai1,2; Wang, Jing1,2; Cui, Yongqing1,2; Zhu, Xiang1,2; Su, Xiang1,2; Zhou, Yuanbao1,2; Zhang, Delin1,2; Tang, Chun3,4; Yin, Ping1,2; Liu, Zhu1,2; Zou, Tingting5
2019-05-28
Source PublicationCELL DISCOVERY
ISSN2056-5968
Volume5Pages:14
AbstractA newly identified arbitrium communication system regulates the lysis-to-lysogeny decision in a Bacillus bacteriophage. This system contains an arbitrium hexapeptide as a signal, the cellular receptor AimR, and the lysogenic negative regulator AimX. AimR specifically targets the downstream DNA to activate aimX gene expression. The arbitrium peptide binds to AimR, inhibiting its DNA-binding to promote phage lysogeny. Recently, we and other groups have elucidated how arbitrium peptide sensed by AimR. However, the molecular mechanisms of DNA recognition by AimR and the regulation of its DNA-binding activity by the peptide remain largely unknown. Here, we report the crystal structure of the AimR-DNA complex at 2.1 angstrom resolution. The N-terminal H T H motif recognizes the palindromic DNA sequence, buttressed by interactions between positively charged residues and the DNA phosphate groups. The DNA-bound AimR assembles a more closed dimer than the peptide-bound form. Single-molecule FRET and crosslinking assays revealed that the AimR protein samples both open and closed conformations in solution. Arbitrium peptide binding induces a closed-to-open conformational change of AimR, eliminating DNA targeting. Our structural and functional analysis provides new insights into the DNA recognition mechanism of AimR and its regulation by the arbitrium peptide in the context of phage lysis-lysogeny decisions.
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
DOI10.1038/s41421-019-0101-2
WOS KeywordMACROMOLECULAR CRYSTALLOGRAPHY BEAMLINE ; TO-CELL COMMUNICATION ; QUORUM ; MODEL ; PLCR
Language英语
Funding ProjectNational Key R&D Program of China[2018YFA0507700] ; National Natural Science Foundation of China[81601/42] ; Fundamental Research Funds for the Central Universities[2662014BQ028] ; Fundamental Research Funds for the Central Universities[2662017PY031]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000471023100001
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/14533
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorZou, Tingting
Affiliation1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China
2.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Hubei, Peoples R China
3.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
4.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
5.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Guan, Zeyuan,Pei, Kai,Wang, Jing,et al. Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage[J]. CELL DISCOVERY,2019,5:14.
APA Guan, Zeyuan.,Pei, Kai.,Wang, Jing.,Cui, Yongqing.,Zhu, Xiang.,...&Zou, Tingting.(2019).Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage.CELL DISCOVERY,5,14.
MLA Guan, Zeyuan,et al."Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage".CELL DISCOVERY 5(2019):14.
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