APM OpenIR
Rapid Discovery of the Potential Toxic Compounds in Polygonum multiflorum by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis
Han, Lifeng1,2; Wang, Piao1; Wang, Yulan2,3; Zhao, Qianyu1; Zheng, Fang1; Dou, Zhiying1; Yang, Wenzhi1; Hu, Limin1; Liu, Caixiang2
2019-04-16
Source PublicationFRONTIERS IN PHARMACOLOGY
ISSN1663-9812
Volume10Pages:12
AbstractThe dry roots of Polygonum multiflorum (PM), involving both the raw and processed materials, are widely used as the traditional Chinese medicine for treating various diseases in China. Hepatotoxicity has been occasionally reported in patients who consume PM. Unfortunately, no definite criteria are currently available regarding the processing technology of PM for reduction the toxicity. In this work, we aimed to investigate the variations of PM metabolite profiles induced by different processing technologies by UHPLC/Q-Orbitrap-MS and multivariate statistical analysis, and to discover the potential toxic compounds by correlating the cytotoxicity of L02 cell with the contents of metabolites in raw and processed PM samples. We could identify two potential toxic compounds, emodin-8-O-glucoside and torachrysone-O-hexose, which could be selected as the toxic markers to evaluate different processing methods. The results indicated all processed PM samples could decrease the cytotoxicity on L02 cell. The best processing technology for PM process was to steam PM in black soybean decoction (BD-PM) for 24 h.
KeywordPolygonum multiflorum metabolomics hepatotoxicity L02 cell UHPLC/Q-Orbitrap-MS
Funding OrganizationImportant Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China
DOI10.3389/fphar.2019.00329
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; TIME-OF-FLIGHT ; ANTIOXIDANT PHENOLIC-COMPOUNDS ; IDIOSYNCRATIC HEPATOTOXICITY ; STILBENE GLUCOSIDE ; ACUTE HEPATITIS ; CONSTITUENTS ; COMPONENTS ; ANTHRAQUINONES ; IDENTIFICATION
Language英语
Funding ProjectImportant Drug Development Fund, Ministry of Science and Technology of China[2015ZX09501004-003-004] ; Program for National Natural Science Foundation of China[81703934]
Funding OrganizationImportant Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Important Drug Development Fund, Ministry of Science and Technology of China ; Program for National Natural Science Foundation of China ; Program for National Natural Science Foundation of China
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000465027700001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/13827
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorWang, Yulan; Liu, Caixiang
Affiliation1.Tianjin Univ Tradit Chinese Med, Tianjin Key Lab TCM Chem & Anal, Tianjin State Key Lab Modern Chinese Med, Tianjin, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan, Hubei, Peoples R China
3.Nanyang Technol Univ, Sch Biol Sci, Lee Kong Chian Sch Med, Singapore Phenome Ctr, Nanyang, Singapore
Recommended Citation
GB/T 7714
Han, Lifeng,Wang, Piao,Wang, Yulan,et al. Rapid Discovery of the Potential Toxic Compounds in Polygonum multiflorum by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis[J]. FRONTIERS IN PHARMACOLOGY,2019,10:12.
APA Han, Lifeng.,Wang, Piao.,Wang, Yulan.,Zhao, Qianyu.,Zheng, Fang.,...&Liu, Caixiang.(2019).Rapid Discovery of the Potential Toxic Compounds in Polygonum multiflorum by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis.FRONTIERS IN PHARMACOLOGY,10,12.
MLA Han, Lifeng,et al."Rapid Discovery of the Potential Toxic Compounds in Polygonum multiflorum by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis".FRONTIERS IN PHARMACOLOGY 10(2019):12.
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