WIPM OpenIR
Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI
Huang, Cheng1; Wang, Jie2,5; Wang, Ning3; Du, Feng3; Xiong, Wei3; Qian, Junchao4; Zhong, Kai4; Cai, Aoling2; Xu, Shijin1; Huang, Jun1; Cheng, Xueying1; He, Shufang1; Zhang, Ye1
2019-06-15
Source PublicationINTERNATIONAL JOURNAL OF CARDIOLOGY
ISSN0167-5273
Volume285Pages:59-64
AbstractBackground: Myocardial ischemia and reperfusion-evoked spinal reflexes involve nociceptive signals that trigger neuronal excitation through cardiac afferents, projecting into the thoracic spinal cord. Ischemic preconditioning (IPC) involves brief episodes of sublethal ischemia and reperfusion enhances the resistance of the myocardium to subsequent ischemic insults. This study investigated the effects of IPC on ischemia-reperfusion (I/R) stimulation induced activation in the thoracic spinal cord of rats. Methods: A new remotely controlled I/R model was established. The infarct size was determined as a percentage of area at risk (IS/AAR) and arrhythmia scores were evaluated. Non-invasive in vivo fMRI was used for signal quantitative analysis of thoracic spinal spatiotemporal. The role of IPC on the excitability of substantia gelatinosa (SG) neurons was assessed by spinal patch clamp recording technique. The altered expressions of c-Fos, SP, and CGRP in T-4 segment were detected by immunohistochemical staining. Results: The novel I/Rmodel was induced successfully and reliably utilized, and IPC treatment markedly reduced the myocardial infarct size. fMRI analysis revealed that IPC reduced the increased BOLD signals induced by prolonged ischemia-reperfusion. Patch clamp recording showed that IPC treatment reversed the enhanced excitability of SG neurons during I/R treatment. The results of immunofluorescent staining indicated that IPC mitigated the I/R-induced dramatic increase of c-Fos, and reduced the release of SP and CGRP in dorsal horns of spinal cord. Conclusions: These results suggested that IPC suppressed neuronal activation induced by I/R stimuli in rat thoracic spinal cord using spinal cord fMRI and patch clamp recording techniques. (C) 2019 Elsevier B.V. All rights reserved.
KeywordFunctional MRI Myocardial ischemia-reperfusion injury Substantia gelatinosa Spinal cord Patch clamp recording
Funding OrganizationNatural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China
DOI10.1016/j.ijcard.2019.03.025
WOS KeywordSUBSTANCE-P RELEASE ; GELATINOSA NEURONS ; CARDIAC ISCHEMIA ; AFFERENT ; BRADYKININ ; BOLD ; FMRI ; EXCITABILITY ; MODULATION ; MECHANISMS
Language英语
Funding ProjectNatural Science Foundation of Higher Education Institutions of Anhui Province, China[KJ2014ZD16] ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China[KJ2017A172] ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China[Y6Y0021004]
Funding OrganizationNatural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China
WOS Research AreaCardiovascular System & Cardiology
WOS SubjectCardiac & Cardiovascular Systems
WOS IDWOS:000464016000011
PublisherELSEVIER IRELAND LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/13726
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorHe, Shufang; Zhang, Ye
Affiliation1.Anhui Med Univ, Dept Anesthesiol, Hosp 2, 678 Furong Rd, Hefei 230601, Anhui, Peoples R China
2.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst,Wuhan Inst Phy, Wuhan 430071, Hubei, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Neurodegenerat Disorder Res Ctr, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China
4.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China
5.Second Hosp Shijiazhuang, Shijiazhuang 050051, Hebei, Peoples R China
Recommended Citation
GB/T 7714
Huang, Cheng,Wang, Jie,Wang, Ning,et al. Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI[J]. INTERNATIONAL JOURNAL OF CARDIOLOGY,2019,285:59-64.
APA Huang, Cheng.,Wang, Jie.,Wang, Ning.,Du, Feng.,Xiong, Wei.,...&Zhang, Ye.(2019).Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI.INTERNATIONAL JOURNAL OF CARDIOLOGY,285,59-64.
MLA Huang, Cheng,et al."Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI".INTERNATIONAL JOURNAL OF CARDIOLOGY 285(2019):59-64.
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