WIPM OpenIR
A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood
Li, Xinyi1,2; Deng, Qiong1,2; Liu, Hongping3; Lei, Youwang4; Fan, Pengwei5; Wang, Bin5; Chen, Yangfei1,2; Smith, Zachary J.6; Tang, Yuchen1,2; Gao, Tingjuan1,2
2019-05-01
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume411Issue:13Pages:2767-2780
AbstractBlood counting is one of the most commonly ordered clinical assays, and is often part of the basis for initial diagnosis and screening for disease. While substantial prior research has shown the ability of portable instruments to accurately produce blood counts through image- or flow-based cytometry, these methods require complex sample preparation using either costly commercial imaging chambers or complicated reagents. To address these issues, in this paper we developed a method to prepare trace volumes of whole blood aimed at portable blood counting. The strategy is based on pre-storing dry-form reagents and fabricating a specifically designed cell counter. In order to obtain total cell counts for red blood cells, platelets, and 3-part differentials of white blood cells, two parallel counting chambers with different depths are made from cost- and environmentally friendly materials using soft lithography. As little as 1l of whole blood is prepared with pre-stored reagents in centrifuge vials, whereas red blood cells are sphered and white blood cells are stained at the same time. Driven by the capillary force, prepared blood samples enter the hydrophilic chambers automatically. Monolayers of cells are formed when the blood dilution factors and the chamber depths are co-optimized. Combined with our previous custom-built instrument and automated analysis algorithm, the sample preparation strategy allows producing counting results with excellent agreement to a gold-standard clinical hematology instrument. The success of this preparation method may further advance applications of our technology for global use in low-resource settings where central hematology laboratories are not accessible.
KeywordBlood counting POCT Image-based cytometry Sample preparation
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China
DOI10.1007/s00216-019-01738-w
WOS KeywordDYE ; COMPACT
Language英语
Funding ProjectNational Natural Science Foundation of China[21605054] ; Central China Normal University (CCNU)[210-31102] ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE)[234-20205016002] ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE)[234-20205170355] ; Program of Introducing Talents of Discipline to University of China (111 program)[B17019] ; CCNU Program of Innovation and Entrepreneurship for college students[220-20205180419] ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China[2016YFA020130]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000468133600004
PublisherSPRINGER HEIDELBERG
Citation statistics
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/13636
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorTang, Yuchen; Gao, Tingjuan
Affiliation1.Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei, Peoples R China
2.Cent China Normal Univ, Coll Chem, Chem Biol Ctr, Wuhan 430079, Hubei, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
4.Jieyang Women & Childrens Hlth Care Hosp, Prenatal Diagnost Ctr, Jieyang 522000, Guangdong, Peoples R China
5.Wuhan 7 Hosp, Clin Lab Ctr, Wuhan 430071, Hubei, Peoples R China
6.Univ Sci & Technol China, Dept Precis Machinery & Precis Instrumentat, Hefei 230027, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Li, Xinyi,Deng, Qiong,Liu, Hongping,et al. A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(13):2767-2780.
APA Li, Xinyi.,Deng, Qiong.,Liu, Hongping.,Lei, Youwang.,Fan, Pengwei.,...&Gao, Tingjuan.(2019).A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(13),2767-2780.
MLA Li, Xinyi,et al."A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.13(2019):2767-2780.
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