WIPM OpenIR
Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy
Ye, Yansheng1; Wu, Qiong1; Zheng, Wenwen1; Jiang, Bin1; Pielak, Gary J.2,3; Liu, Maili1; Li, Conggang1
2018
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume410Issue:3Pages:869-874
SubtypeArticle
AbstractProtein diffusion in living cells might differ significantly from that measured in vitro. Little is known about the effect of globular protein size on rotational diffusion in cells because each protein has distinct surface properties, which result in different interactions with cellular components. To overcome this problem, the B1 domain of protein G (GB1) and several concatemers of the protein were labeled with 5-fluorotryptophan and studied by F-19 NMR in Escherichia coli cells, Xenopus laevis oocytes, and in aqueous solutions crowded with glycerol, or Ficoll70 (TM) and lysozyme. Relaxation data show that the size dependence of protein rotation in cells is due to weak interactions of the target protein with cellular components, but the effect of these interactions decreases as protein size increases. The results provide valuable information for interpreting protein diffusion data acquired in living cells.
KeywordProteins Rotational Motion Nmr Viscosity Weak Interactions
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationMinistry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854)
DOI10.1007/s00216-017-0745-4
WOS KeywordMAGNETIC-RESONANCE-SPECTROSCOPY ; XENOPUS-LAEVIS OOCYTES ; LIVING CELLS ; ESCHERICHIA-COLI ; MULTIDIMENSIONAL NMR ; QUINARY STRUCTURE ; IN-VIVO ; DIFFUSION ; CYTOPLASM ; ENVIRONMENTS
Indexed BySCI
Language英语
Funding OrganizationMinistry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000422952600017
PublisherSPRINGER HEIDELBERG
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/12819
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorLiu, Maili; Li, Conggang
Affiliation1.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China
2.Univ North Carolina Chapel Hill, Dept Chem Biochem & Biophys, Chapel Hill, NC 27599 USA
3.Univ North Carolina Chapel Hill, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA
Recommended Citation
GB/T 7714
Ye, Yansheng,Wu, Qiong,Zheng, Wenwen,et al. Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(3):869-874.
APA Ye, Yansheng.,Wu, Qiong.,Zheng, Wenwen.,Jiang, Bin.,Pielak, Gary J..,...&Li, Conggang.(2018).Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(3),869-874.
MLA Ye, Yansheng,et al."Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.3(2018):869-874.
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