APM OpenIR
Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana
Chen, Fangfang1,2,3; Liu, Caixiang4; Zhang, Jingtao4; Lei, Hehua4; Li, He-Ping2,3; Liao, Yu-Cai2,3; Tang, Huiru4,5,6
2018-01-04
Source PublicationFRONTIERS IN PLANT SCIENCE
ISSN1664-462X
Volume8
SubtypeArticle
AbstractFusarium head blight disease resulting from Fusarium graminearum (FG) infection causes huge losses in global production of cereals and development of FG-resistant plants is urgently needed. To understand biochemistry mechanisms for FG resistance, here, we have systematically investigated the plant metabolomic phenotypes associated with FG resistance for transgenic Arabidopsis thaliana expressing a class-I chitinase (Chi), a Fusarium-specific recombinant antibody gene (CWP2) and fused Chi-CWP2. Plant disease indices, mycotoxin levels, metabonomic characteristics, and expression levels of several key genes were measured together with their correlations. We found that A. thaliana expressing Chi-CWP2 showed higher FG resistance with much lower disease indices and mycotoxin levels than the wild-type and the plants expressing Chi or CWP2 alone. The combined metabonomic and quantitative RT-PCR analyses revealed that such FG-resistance was closely associated with the promoted biosynthesis of secondary metabolites (phenylpropanoids, alkanoids) and organic osmolytes (proline, betaine, glucose, myo-inositol) together with enhanced TCA cycle and GABA shunt. These suggest that the concurrently enhanced biosyntheses of the shikimate-mediated secondary metabolites and organic osmolytes be an important strategy for A. thaliana to develop and improve FG resistance. These findings provide essential biochemical information related to FG resistance which is important for developing FG-resistant cereals.
KeywordArabidopsis Thaliana Fusarium Graminearum Metabolic Responses Mycotoxins Chi-cwp2 Nmr
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004) ; National Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004) ; National Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004) ; National Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004)
DOI10.3389/fpls.2017.02177
WOS KeywordANTIBODY-MEDIATED RESISTANCE ; MULTIVARIATE DATA-ANALYSIS ; THAUMATIN-LIKE PROTEIN ; HEAD BLIGHT ; H-1-NMR SPECTROSCOPY ; FUNGAL PATHOGENS ; TRICHOTHECENE BIOSYNTHESIS ; FUTURE-PROSPECTS ; PLANT-PATHOGENS ; WHEAT PLANTS
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004) ; National Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004) ; National Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004) ; National Natural Science Foundation of China(31600240 ; National Natural Science Foundation of China(31600240 ; 31200208 ; 31200208 ; 21175149 ; 21175149 ; 31272004) ; 31272004)
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000419330100001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.apm.ac.cn/handle/112942/12803
Collection中国科学院武汉物理与数学研究所
Corresponding AuthorLiao, Yu-Cai; Tang, Huiru
Affiliation1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China
3.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China
4.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, CAS Key Lab Magnet Resonance Biol Syst,State Key, Wuhan, Hubei, Peoples R China
5.Fudan Univ, State Key Lab Genet Engn, Zhongshan Hosp, Shanghai, Peoples R China
6.Fudan Univ, Sch Life Sci, Collaborat Innovat Ctr Genet & Dev, Shanghai Int Ctr Mol Phen,Metabon & Syst Biol Lab, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Chen, Fangfang,Liu, Caixiang,Zhang, Jingtao,et al. Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana[J]. FRONTIERS IN PLANT SCIENCE,2018,8.
APA Chen, Fangfang.,Liu, Caixiang.,Zhang, Jingtao.,Lei, Hehua.,Li, He-Ping.,...&Tang, Huiru.(2018).Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana.FRONTIERS IN PLANT SCIENCE,8.
MLA Chen, Fangfang,et al."Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana".FRONTIERS IN PLANT SCIENCE 8(2018).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Fangfang]'s Articles
[Liu, Caixiang]'s Articles
[Zhang, Jingtao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Fangfang]'s Articles
[Liu, Caixiang]'s Articles
[Zhang, Jingtao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Fangfang]'s Articles
[Liu, Caixiang]'s Articles
[Zhang, Jingtao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.