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An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain
He, Wei-Jie1,2; Zhang, Limin3; Yi, Shu-Yuan1,4; Tang, Xue-Ling1,4; Yuan, Qing-Song1,4; Guo, Mao-Wei1,4; Wu, Ai-Bo1,5; Qu, Bo1,4; Li, He-Ping1,2; Liao, Yu-Cai1,4,6
2017-08-25
Source PublicationSCIENTIFIC REPORTS
Volume7
SubtypeArticle
AbstractDegradation of toxins by microorganisms is a promising approach for detoxification of agricultural products. Here, a bacterial strain, Sphingomonas S3-4, that has the ability to degrade the mycotoxin deoxynivalenol (DON) was isolated from wheat fields. Incubation of Fusarium-infected wheat grains with S3-4 completely eliminated DON. In S3-4 DON is catabolized into compounds with no detectable phytotoxicity, 3-oxo-DON and 3-epi-DON, via two sequential reactions. Comparative analysis of genome sequences from two DON-degrading strains, S3-4 and Devosia D17, and one non-DON-degrading strain, Sphingobium S26, combined with functional screening of a S3-4 genomic BAC library led to the discovery that a novel aldo/keto reductase superfamily member, AKR18A1, is responsible for oxidation of DON into 3-oxo-DON. DON-degrading activity is completely abolished in a mutant S3-4 strain where the AKR18A1 gene is disrupted. Recombinant AKR18A1 protein expressed in Escherichia coli catalyzed the reversible oxidation/reduction of DON at a wide range of pH values (7.5 to 11) and temperatures (10 to 50 degrees C). The S3-4 strain and recombinant AKR18A1 also catabolized zearalenone and the aldehydes glyoxal and methyglyoxal. The S3-4 strain and the AKR18A1 gene are promising agents for the control of Fusarium pathogens and detoxification of mycotoxins in plants and in food/feed products.
WOS HeadingsScience & Technology
Funding OrganizationNational Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; National Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001)
DOI10.1038/s41598-017-08799-w
WOS KeywordHEAD BLIGHT ; MYCOTOXIN DEOXYNIVALENOL ; ESCHERICHIA-COLI ; RNA GENES ; DETOXIFICATION ; WHEAT ; DEGRADATION ; RESISTANCE ; SUPERFAMILY ; ANNOTATION
Indexed BySCI
Language英语
Funding OrganizationNational Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; National Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001)
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000408449000028
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/11378
Collection磁共振应用研究部
Affiliation1.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Food Safety, Res Inst Nutr Sci, Shanghai 200031, Peoples R China
6.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
He, Wei-Jie,Zhang, Limin,Yi, Shu-Yuan,et al. An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain[J]. SCIENTIFIC REPORTS,2017,7.
APA He, Wei-Jie.,Zhang, Limin.,Yi, Shu-Yuan.,Tang, Xue-Ling.,Yuan, Qing-Song.,...&Liao, Yu-Cai.(2017).An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain.SCIENTIFIC REPORTS,7.
MLA He, Wei-Jie,et al."An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain".SCIENTIFIC REPORTS 7(2017).
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