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High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats
An, Yanpeng1,3; Xu, Wenxin1,3; Li, Huihui1,3; Lei, Hehua1; Zhang, Limin1; Hao, Fuhua1; Duan, Yixuan1,4; Yan, Xing1,3; Zhao, Ying1,4; Wu, Junfang1; Wang, Yulan1,2; Tang, Huiru1
2013-08-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
Volume12Issue:8Pages:3755-3768
SubtypeArticle
AbstractObesity is a condition resulting from the interactions of individual biology and environmental factors causing multiple complications. To understand the system's metabolic changes associated with the obesity development and progression, we systematically analyzed the dynamic metabonomic changes induced by a high-fat diet (HFD) in multiple biological matrices of rats using NMR and GC-FID/MS techniques. Clinical chemistry and histopathological data were obtained as complementary information. We found that HFD intakes caused systematic metabolic changes in blood plasma, liver, and urine samples involving multiple metabolic pathways including glycolysis, TCA cycle, and gut microbiota functions together with the metabolisms of fatty adds, amino adds, choline, B-vitamins, purines, and pyrimidines. The HFD-induced metabolic variations were detectable in rat urine a week after HFD intake and showed clear dependence on the intake duration. B-vitamins and gut microbiota played important roles in the obesity development and progression together with changes in TCA cycle intermediates (citrate, a-ketoglutarate, succinate, and fumarate). 83-day HFD intakes caused significant metabolic alterations in rat liver highlighted with the enhancements in lipogenesis, lipid accumulation and lipid oxidation, suppression of glycolysis, up-regulation of gluconeogenesis and glycogenesis together with altered metabolisms of choline, amino acids and nucleotides. HFD intakes reduced the PUFA-to-MUFA ratio in both plasma and liver, indicating the HFD-induced oxidative stress. These findings provided essential biochemistry information about the dynamic metabolic responses to the development and progression of HFD-induced obesity. This study also demonstrated the combined metabonomic analysis of multiple biological matrices as a powerful approach for understanding the molecular basis of pathogenesis and disease progression.
KeywordObesity Metabolism High-fat Diet Nmr Multivariate Data Analysis
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1021/pr400398b
WOS KeywordINSULIN-RESISTANT MICE ; HUMAN-BLOOD-PLASMA ; H-1-NMR SPECTROSCOPY ; GUT MICROBIOTA ; OBESITY ; LIVER ; ACID ; SYSTEMS ; STRESS ; METABONOMICS
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000322852800019
Citation statistics
Cited Times:75[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/1034
Collection磁共振应用研究部
Affiliation1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
2.Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
An, Yanpeng,Xu, Wenxin,Li, Huihui,et al. High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(8):3755-3768.
APA An, Yanpeng.,Xu, Wenxin.,Li, Huihui.,Lei, Hehua.,Zhang, Limin.,...&Tang, Huiru.(2013).High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats.JOURNAL OF PROTEOME RESEARCH,12(8),3755-3768.
MLA An, Yanpeng,et al."High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats".JOURNAL OF PROTEOME RESEARCH 12.8(2013):3755-3768.
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