WIPM OpenIR  > 磁共振应用研究部
High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats
An, Yanpeng1,3; Xu, Wenxin1,3; Li, Huihui1,3; Lei, Hehua1; Zhang, Limin1; Hao, Fuhua1; Duan, Yixuan1,4; Yan, Xing1,3; Zhao, Ying1,4; Wu, Junfang1; Wang, Yulan1,2; Tang, Huiru1
AbstractObesity is a condition resulting from the interactions of individual biology and environmental factors causing multiple complications. To understand the system's metabolic changes associated with the obesity development and progression, we systematically analyzed the dynamic metabonomic changes induced by a high-fat diet (HFD) in multiple biological matrices of rats using NMR and GC-FID/MS techniques. Clinical chemistry and histopathological data were obtained as complementary information. We found that HFD intakes caused systematic metabolic changes in blood plasma, liver, and urine samples involving multiple metabolic pathways including glycolysis, TCA cycle, and gut microbiota functions together with the metabolisms of fatty adds, amino adds, choline, B-vitamins, purines, and pyrimidines. The HFD-induced metabolic variations were detectable in rat urine a week after HFD intake and showed clear dependence on the intake duration. B-vitamins and gut microbiota played important roles in the obesity development and progression together with changes in TCA cycle intermediates (citrate, a-ketoglutarate, succinate, and fumarate). 83-day HFD intakes caused significant metabolic alterations in rat liver highlighted with the enhancements in lipogenesis, lipid accumulation and lipid oxidation, suppression of glycolysis, up-regulation of gluconeogenesis and glycogenesis together with altered metabolisms of choline, amino acids and nucleotides. HFD intakes reduced the PUFA-to-MUFA ratio in both plasma and liver, indicating the HFD-induced oxidative stress. These findings provided essential biochemistry information about the dynamic metabolic responses to the development and progression of HFD-induced obesity. This study also demonstrated the combined metabonomic analysis of multiple biological matrices as a powerful approach for understanding the molecular basis of pathogenesis and disease progression.
KeywordObesity Metabolism High-fat Diet Nmr Multivariate Data Analysis
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000322852800019
Citation statistics
Cited Times:75[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
2.Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
An, Yanpeng,Xu, Wenxin,Li, Huihui,et al. High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(8):3755-3768.
APA An, Yanpeng.,Xu, Wenxin.,Li, Huihui.,Lei, Hehua.,Zhang, Limin.,...&Tang, Huiru.(2013).High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats.JOURNAL OF PROTEOME RESEARCH,12(8),3755-3768.
MLA An, Yanpeng,et al."High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats".JOURNAL OF PROTEOME RESEARCH 12.8(2013):3755-3768.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[An, Yanpeng]'s Articles
[Xu, Wenxin]'s Articles
[Li, Huihui]'s Articles
Baidu academic
Similar articles in Baidu academic
[An, Yanpeng]'s Articles
[Xu, Wenxin]'s Articles
[Li, Huihui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[An, Yanpeng]'s Articles
[Xu, Wenxin]'s Articles
[Li, Huihui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.