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Confinement Alters the Structure and Function of Calmodulin
Xu, Guohua1; Cheng, Kai1,2; Wu, Qiong1; Liu, Maili1; Li, Conggang1
2017-01-09
Source PublicationANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume56Issue:2Pages:530-534
SubtypeArticle
AbstractMany cellular reactions involving proteins, including their biosynthesis, misfolding, and transport, occur in confined compartments. Despite its importance, a structural basis of understanding of how confined environments alter protein function is still lacking. Herein, we explore structure-function correlations of calmodulin (CaM), a multidomain protein involved in many calcium-mediated signaling pathways, in reverse micelles. Confinement dramatically alters CaM structure and function. The protein forms an extended structure in bulk water, but becomes compacted in reverse micelles. In addition, confinement changes the function of CaM. Specifically, the protein binds the MLCK, AcN19, and somatostatin peptides in dilute buffer, but binds only the MLCK and AcN19 peptides in reverse micelles. In summary, we determined a new CaM structure in reverse micelles and demonstrate that confinement can modulate both protein structure and function.
KeywordCalmodulin Confinement Nmr Spectroscopy Protein Structures Reverse Micelles
WOS HeadingsScience & Technology ; Physical Sciences
Funding OrganizationMinistry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; 21173258 ; 21173258 ; 21120102038 ; 21120102038 ; 21221064) ; 21221064) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; 21173258 ; 21173258 ; 21120102038 ; 21120102038 ; 21221064) ; 21221064)
DOI10.1002/anie.201609639
WOS KeywordMOLECULAR-STRUCTURE DETERMINATION ; REVERSE MICELLES ; XPLOR-NIH ; ENCAPSULATED PROTEINS ; BACKBONE DYNAMICS ; NMR-SPECTROSCOPY ; PARAMAGNETIC NMR ; LOW-TEMPERATURE ; UBIQUITIN ; SPECTRA
Indexed BySCI
Language英语
Funding OrganizationMinistry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; 21173258 ; 21173258 ; 21120102038 ; 21120102038 ; 21221064) ; 21221064) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21575156 ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; 21173258 ; 21173258 ; 21120102038 ; 21120102038 ; 21221064) ; 21221064)
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000394996100013
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.wipm.ac.cn/handle/112942/10027
Collection磁共振基础研究部
Affiliation1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst,Collaborat Inn, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100029, Peoples R China
Recommended Citation
GB/T 7714
Xu, Guohua,Cheng, Kai,Wu, Qiong,et al. Confinement Alters the Structure and Function of Calmodulin[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(2):530-534.
APA Xu, Guohua,Cheng, Kai,Wu, Qiong,Liu, Maili,&Li, Conggang.(2017).Confinement Alters the Structure and Function of Calmodulin.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(2),530-534.
MLA Xu, Guohua,et al."Confinement Alters the Structure and Function of Calmodulin".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.2(2017):530-534.
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